Comparison of Radiography, Ultrasonography and SNAP cPL in the Diagnosis of Acute Pancreatitis in Dogs

The study was aimed to compare various diagnostic modalities in the diagnosis of acute pancreatitis in dogs. Forty dogs with history of acute onset of vomiting, abdominal pain and anorexia were screened for acute pancreatitis by radiography, abdominal ultrasound and confirmed by SNAP cPL (Canine pancreatic lipase) kit test. Fourteen (0.375 per cent of gastrointestinal cases) dogs found to be positive for acute pancreatitis by abdominal ultrasound were selected and further subjected to radiography to rule out the underlying causes of acute pancreatitis and confirmed by SNAP cPL test. Out of fourteen dogs, four dogs (29 per cent) having specific radiographic changes gas filled intestinal loops with loss of serosal details, SNAP cPL was positive in nine dogs (64 per cent). Abdominal ultrasonography revealed hypoechoic pancreas, hyperechoic surrounding fat saponification, enlarged or irregular pancreas and dilatation of biliary or pancreatic duct as the major changes in acute pancreatitis dogs. Ultrasound was found to be superior to radiography in the diagnosis of acute pancreatitis and SNAP cPL was found to be effective in confirming only in acute stages of pancreatitis

Análise da marcação de células da linhagem C6 de glioma com as lectinas vegetais CPL, WGA, e Con A / cell marking analysis of C6 glioma line with plant lectins CPL, WGA and Con A

Introdução e objetivos: O glioblastoma multiforme é um glioma de alto grau que apresenta um prognóstico ruim. O diagnóstico definitivo é estabelecido pela avaliação histológica, porém este pode apresentar conflitos na classificação, com isso surge à necessidade de ferramentas que auxiliem o patologista em sua análise. Atualmente, maior ênfase tem sido dada a alterações na glicosilação, pois estão associadas a neoplasias, e a descoberta da capacidade de lectinas em reconhecer tais alterações fez destas, ferramentas aplicáveis para o diagnóstico biomédico. Dessa forma, o objetivo deste trabalho é analisar a marcação das lectinas CpL, WGA e Con A em células da linhagem C6...

Introduction and objectives: Glioblastoma multiforme is a high-grade glioma that has a poor prognosis. The definitive diagnosis is established by histological assessment. However, this can present conflicts in grading gliomas, which justifies new tools to assist the pathologist in his analysis. Currently, it is known that there are changes in glycosylation pattern of molecules associated with cancer, and the discovery of the ability of lectins to recognize these changes made these tools applicable for biomedical diagnosis. Thus, the aim of this study is to analyze the labelling of C6...